Calibration of micrograph image's dimension
This refers to micrographs taken with the CoolSNAP Pro CCD camera in our lab.
The CCD used in the CoolSNAP Pro color camera has a 1/2" format, which means the size of the CCD is: 6.4mm x 4.8 mm
So if the 5x objective is used, the micrograph's physical dimension is:
(dimension of CCD) / 5
or
1.28 mm x 0.96 mm
Similarly, with 10x objective, the micrograph's physical dimension is:
0.64 mm x 0.48 mm
These values can be used to calibrate the dimensions of micrographs taken by this camera.
------------------------------------------------------
L-shaped lens in the lab:
magnification: 1.5x, 2.0x, and zoom lens 0.7x - 4.55x
Q-imaging 1394 firewire CCD camera: 1/2" CCD (6.4 mm x 4.8 mm)
Resolution: 1392 x 1040
L-shaped lens with 1/2" CCD:
zoom lens at 4.55x: physical dimension of the image is 468.86 micron in length and 351.65 micron in width
zoom lens at 0.7x: image dimension is 3047.6 micron x 2285.7 micron
For calibration at 4.55x: 468.86 micron/1392 pixel = 0.3368 micron/pixel
For calibration at 0.7 x: 3047.6 micron/1392 pixel = 2.1894 micron/pixel


0 Comments:
Post a Comment
<< Home