Tuesday, April 11, 2006

Calibration of micrograph image's dimension

This refers to micrographs taken with the CoolSNAP Pro CCD camera in our lab.

The CCD used in the CoolSNAP Pro color camera has a 1/2" format, which means the size of the CCD is: 6.4mm x 4.8 mm
So if the 5x objective is used, the micrograph's physical dimension is:
(dimension of CCD) / 5
or
1.28 mm x 0.96 mm

Similarly, with 10x objective, the micrograph's physical dimension is:
0.64 mm x 0.48 mm

These values can be used to calibrate the dimensions of micrographs taken by this camera.

------------------------------------------------------
L-shaped lens in the lab:
magnification: 1.5x, 2.0x, and zoom lens 0.7x - 4.55x

Q-imaging 1394 firewire CCD camera: 1/2" CCD (6.4 mm x 4.8 mm)
Resolution: 1392 x 1040

L-shaped lens with 1/2" CCD:
zoom lens at 4.55x: physical dimension of the image is 468.86 micron in length and 351.65 micron in width
zoom lens at 0.7x: image dimension is 3047.6 micron x 2285.7 micron

For calibration at 4.55x: 468.86 micron/1392 pixel = 0.3368 micron/pixel
For calibration at 0.7 x: 3047.6 micron/1392 pixel = 2.1894 micron/pixel

0 Comments:

Post a Comment

<< Home